Journal: Foods

Article Title: Citri grandis Exocarpium Extract Alleviates Atherosclerosis in ApoE −/− Mice by Modulating the Expression of TGF-β1, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1

doi: 10.3390/foods14244267

Figure Lengend Snippet: Representative blotting images and protein densitometry showing the relative expression levels of TGF-β, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1 proteins normalized with GAPDH and β-actin in aorta of apoE −/− mice . ( A , E ) are the representative protein blotting images; ( B – D , F – H ) Expression levels of TGF-β, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1 proteins in aorta were shown in the bar chart and the blotting grayscale value analyses normalized with GAPDH and β-actin (Wilks’ Λ = 0.003, R 2 = 0.970, Eta 2 = 0.970), which were used as loading control. The error bars represented x ¯ ± SE. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the MOD group indicate a statistically significant difference; n = at least 3 for each group.

Article Snippet: Polyclonal antibodies against PI3K p85 (rabbit) were obtained from Affinity Biosciences Co., Ltd. (Liyang, Jiangsu, China).

Techniques: Expressing, Control

Journal: Foods

Article Title: Citri grandis Exocarpium Extract Alleviates Atherosclerosis in ApoE −/− Mice by Modulating the Expression of TGF-β1, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1

doi: 10.3390/foods14244267

Figure Lengend Snippet: Effect of CGE on the expression of TGF-β, PI3K, AKT, PPAR-γ, LXR-α, and ABCA1 in mouse livers . ( A – F ) represent the mRNA expression levels of TGF-β, PI3K, AKT1, PPAR-γ, LXR-α, and ABCA1 to GAPDH in livers, respectively (Wilks’ Λ = 0.001, R 2 = 0.953, Eta 2 = 0.958). ( G , K ) are the representative protein blotting images; ( H – J , L – N ) are the respective optical density ratios of bands, which represent the relative expression levels of TGF-β, PI3K, AKT, PPAR-γ, LXR-α, and ABCA1 proteins normalized with GAPDH or β-actin in the livers of apoE −/− mice (Wilks’ Λ = 0.008, R 2 = 0.986, Eta 2 = 0.986). The blotting grayscale value was analyzed relative to the GAPDH band and β-actin, as a loading control. The error bars represent the x ¯ ± SE. It is considered to have statistically significant differences when * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the MOD group; n = at least 3 for each group.

Article Snippet: Polyclonal antibodies against PI3K p85 (rabbit) were obtained from Affinity Biosciences Co., Ltd. (Liyang, Jiangsu, China).

Techniques: Expressing, Control

Journal: Journal of pediatric surgery

Article Title: Spatiotemporal alteration in phosphatidylinositide 3-kinase–serine/threonine protein kinase B signaling in the nitrofen-induced hypoplastic lung

doi: 10.1016/j.jpedsurg.2009.10.075

Figure Lengend Snippet: Relative mRNA expression levels of PI3K in lung (n = 8 for each group at each time-point)

Article Snippet: Sections were incubated overnight at 4°C with each of the primary antibodies including a 1:100 dilution of rabbit polyclonal antibodies against PI3K p110 α (Lot sc-7174; Santa Cruz Biotechnology) and AKT1/2/3 (Lot sc-8312; Santa Cruz Biotechnology).

Techniques: Expressing, Control

Journal: Journal of pediatric surgery

Article Title: Spatiotemporal alteration in phosphatidylinositide 3-kinase–serine/threonine protein kinase B signaling in the nitrofen-induced hypoplastic lung

doi: 10.1016/j.jpedsurg.2009.10.075

Figure Lengend Snippet: PI3K immunohistochemistry. Pulmonary expression of PI3K protein at D15 control (A) and CDH (B) showing no difference in immunoreactivity and distribution. At D18, PI3K expression is diminished in the distal epithelium in CDH lungs (D) compared to control lungs (C). At D21, PI3K immunoreactivity is decreased in the overall intensity in CDH lungs (F), whereas strong PI3K protein expression is seen in control lungs (E).

Article Snippet: Sections were incubated overnight at 4°C with each of the primary antibodies including a 1:100 dilution of rabbit polyclonal antibodies against PI3K p110 α (Lot sc-7174; Santa Cruz Biotechnology) and AKT1/2/3 (Lot sc-8312; Santa Cruz Biotechnology).

Techniques: Immunohistochemistry, Expressing, Control

Journal: Journal of pediatric surgery

Article Title: Spatiotemporal alteration in phosphatidylinositide 3-kinase–serine/threonine protein kinase B signaling in the nitrofen-induced hypoplastic lung

doi: 10.1016/j.jpedsurg.2009.10.075

Figure Lengend Snippet: Primers for quantitative real time reverse transcription polymerase chain reaction

Article Snippet: Sections were incubated overnight at 4°C with each of the primary antibodies including a 1:100 dilution of rabbit polyclonal antibodies against PI3K p110 α (Lot sc-7174; Santa Cruz Biotechnology) and AKT1/2/3 (Lot sc-8312; Santa Cruz Biotechnology).

Techniques: Reverse Transcription, Sequencing

Journal: Mediators of Inflammation

Article Title: A 3 Adenosine Receptor Allosteric Modulator Induces an Anti-Inflammatory Effect: In Vivo Studies and Molecular Mechanism of Action

doi: 10.1155/2014/708746

Figure Lengend Snippet: LUF6000 downregulates the NF- κ B signaling pathway via the A 3 AR. Protein analysis was performed using Western blot (WB) analysis in both PAW and PBMCs. LUF6000 downregulates A 3 AR expression levels in both PAW and PBMCs (a). PI3K, IKK, I κ B, and NF- κ B, all members of the NF- κ B signaling pathway, were downregulated upon LUF6000 administration (b).

Article Snippet: Rabbit polyclonal antibodies against rat A 3 AR, phosphoinositide 3-kinase (PI3K), I κ B kinase (IKK), I κ B, nuclear factor kappa B (NF- κ B), Janus kinase 2 (Jak-2), and signal transducer and activator transcription 1 (STAT-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β -actin were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Western Blot, Expressing