Journal: Journal of pediatric surgery

Article Title: Spatiotemporal alteration in phosphatidylinositide 3-kinase–serine/threonine protein kinase B signaling in the nitrofen-induced hypoplastic lung

doi: 10.1016/j.jpedsurg.2009.10.075

Figure Lengend Snippet: Relative mRNA expression levels of PI3K in lung (n = 8 for each group at each time-point)

Article Snippet: Sections were incubated overnight at 4°C with each of the primary antibodies including a 1:100 dilution of rabbit polyclonal antibodies against PI3K p110 α (Lot sc-7174; Santa Cruz Biotechnology) and AKT1/2/3 (Lot sc-8312; Santa Cruz Biotechnology).

Techniques: Expressing, Control

Journal: Journal of pediatric surgery

Article Title: Spatiotemporal alteration in phosphatidylinositide 3-kinase–serine/threonine protein kinase B signaling in the nitrofen-induced hypoplastic lung

doi: 10.1016/j.jpedsurg.2009.10.075

Figure Lengend Snippet: PI3K immunohistochemistry. Pulmonary expression of PI3K protein at D15 control (A) and CDH (B) showing no difference in immunoreactivity and distribution. At D18, PI3K expression is diminished in the distal epithelium in CDH lungs (D) compared to control lungs (C). At D21, PI3K immunoreactivity is decreased in the overall intensity in CDH lungs (F), whereas strong PI3K protein expression is seen in control lungs (E).

Article Snippet: Sections were incubated overnight at 4°C with each of the primary antibodies including a 1:100 dilution of rabbit polyclonal antibodies against PI3K p110 α (Lot sc-7174; Santa Cruz Biotechnology) and AKT1/2/3 (Lot sc-8312; Santa Cruz Biotechnology).

Techniques: Immunohistochemistry, Expressing, Control

Journal: Journal of pediatric surgery

Article Title: Spatiotemporal alteration in phosphatidylinositide 3-kinase–serine/threonine protein kinase B signaling in the nitrofen-induced hypoplastic lung

doi: 10.1016/j.jpedsurg.2009.10.075

Figure Lengend Snippet: Primers for quantitative real time reverse transcription polymerase chain reaction

Article Snippet: Sections were incubated overnight at 4°C with each of the primary antibodies including a 1:100 dilution of rabbit polyclonal antibodies against PI3K p110 α (Lot sc-7174; Santa Cruz Biotechnology) and AKT1/2/3 (Lot sc-8312; Santa Cruz Biotechnology).

Techniques: Reverse Transcription, Sequencing

Journal: Mediators of Inflammation

Article Title: A 3 Adenosine Receptor Allosteric Modulator Induces an Anti-Inflammatory Effect: In Vivo Studies and Molecular Mechanism of Action

doi: 10.1155/2014/708746

Figure Lengend Snippet: LUF6000 downregulates the NF- κ B signaling pathway via the A 3 AR. Protein analysis was performed using Western blot (WB) analysis in both PAW and PBMCs. LUF6000 downregulates A 3 AR expression levels in both PAW and PBMCs (a). PI3K, IKK, I κ B, and NF- κ B, all members of the NF- κ B signaling pathway, were downregulated upon LUF6000 administration (b).

Article Snippet: Rabbit polyclonal antibodies against rat A 3 AR, phosphoinositide 3-kinase (PI3K), I κ B kinase (IKK), I κ B, nuclear factor kappa B (NF- κ B), Janus kinase 2 (Jak-2), and signal transducer and activator transcription 1 (STAT-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β -actin were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Western Blot, Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Ginsenoside Rb1 Prevents Dysfunction of Endothelial Cells by Suppressing Inflammatory Response and Apoptosis in the High-Fat Diet Plus Balloon Catheter-Injured Rabbit Model via the G Protein-Coupled Estrogen Receptor-Mediated Phosphatidylinositol 3-Kinases (PI3K)/Akt Pathway

doi: 10.12659/MSM.912986

Figure Lengend Snippet: Administration of Rb1 activated GPER/PI3K/Akt axis dysfunctioned in ECs. ECs were isolated from AS and health rabbits and administrated with Rb1 of 20, 40, and 80 μM for 24 h. ( A ) Representative images and quantitative analysis of western blotting detection of molecule expressions in GPER/PI3K/Akt axis. ( B ) Representative images of immunofluorescence detection of GPER expression and distribution. Magnification, 400×. * P <0.05 vs. Control group. # P <0.05 vs. HCD group. Each assay was performed 5 times.

Article Snippet: Antibodies against PI3K (bs-2067R) and phosphorylated PI3K (p-PI3K) (bs-10657R) were purchased from Bioss (Beijing, China).

Techniques: Isolation, Western Blot, Immunofluorescence, Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Ginsenoside Rb1 Prevents Dysfunction of Endothelial Cells by Suppressing Inflammatory Response and Apoptosis in the High-Fat Diet Plus Balloon Catheter-Injured Rabbit Model via the G Protein-Coupled Estrogen Receptor-Mediated Phosphatidylinositol 3-Kinases (PI3K)/Akt Pathway

doi: 10.12659/MSM.912986

Figure Lengend Snippet: Anti-AS function of Rb1 depended on the activation GPER- mediated PI3K/Akt pathway. ECs were isolated from AS and health rabbits and administrated with 80 μM Rb1, 100 nM G-15, and 25 mg/mL 740 Y-P for 24 h. ( A ) Representative images and quantitative analysis of western blotting detection of molecule expressions in GPER/PI3K/Akt axis. ( B ) Representative images and quantitative analysis of flow cytometry detection of apoptosis. ( C ) Quantitative analysis results of ELISA detection of blood IL-6. ( D ) Quantitative analysis results of ELISA detection of blood IL-1β. ( E ) Quantitative analysis results of ELISA detection of blood TNF-α. * P <0.05 vs. HCD+H group. # P <0.05 vs. HCD+H+G-15 group. Each assay was performed 5 times.

Article Snippet: Antibodies against PI3K (bs-2067R) and phosphorylated PI3K (p-PI3K) (bs-10657R) were purchased from Bioss (Beijing, China).

Techniques: Activation Assay, Isolation, Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Citrus unshiu Peel Attenuates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice due to Modulation of the PI3K/Akt Signaling Pathway and MAPK and NF- κ B

doi: 10.1155/2022/4041402

Figure Lengend Snippet: Citrus unshiu peel modulated the PI3K/Akt signaling pathway. Control: control mice, DSS: distilled water administered to ulcerative colitis mice, SASP: sulfasalazine 100 mg/kg body weight administered to ulcerative colitis mice, CUPL: CUP 100 mg/kg body weight administered to ulcerative colitis mice, and CUPH: CUP 200 mg/kg body weight administered to ulcerative colitis mice. Data are mean ± SD ( n = 8). Significance: ### p < 0.001 vs. control mice, ∗∗∗ p < 0.001 vs. DSS mice.

Article Snippet: Rabbit polyclonal antibodies against p-PI3K (#4228), Akt (1 : 1,000, #9272), p-Akt (#9275), and p38 MAPK (#9212) and mouse polyclonal antibodies against c-Jun (1 : 1,000, #2315) were purchased from Cell Signaling Technology, Inc. (Danvers, MN, USA).

Techniques: Control